The Horseracing Integrity & Welfare Unit (HIWU) has instructed the Pennsylvania Equine Toxicology and Research Laboratory (PETRL) to adopt mass spectrometry for total carbon dioxide content testing, aligning it with other program laboratories. This change comes in response to a higher-than-expected incidence of elevated carbon dioxide levels reported by PETRL. Pending cases related to this issue in Pennsylvania have been withdrawn to ensure fairness. HIWU initiated a thorough review involving collaboration with horsemen's representatives and an independent study conducted by the University of California Davis laboratory. The transition underscores the commitment to harmonizing testing methods across the industry.

The decision to standardize on mass spectrometry was driven by discrepancies found between PETRL’s results and those from other laboratories. Initially, PETRL utilized a clinical blood gas analyzer, which had long been accepted by the horseracing industry. However, data from comparative studies indicated that PETRL's outcomes did not align with other labs using mass spectrometry. To address this, HIWU mandated the new methodology as the industry standard. This shift is expected to enhance consistency and reliability in testing for total carbon dioxide content, a critical parameter in equine performance regulation.

The transition process involved extensive evaluation and collaboration. HIWU worked closely with stakeholders, including horsemen’s associations, to ensure transparency and address concerns. An administration study was conducted to compare methodologies, revealing significant differences in test results. Given the rapid degradation of carbon dioxide samples, previous analyses could not be retested. Consequently, HIWU decided to withdraw all pending cases originating from Pennsylvania. For already resolved cases, HISA offered restitution measures such as returning purse money and removing penalties from trainers’ records.

This development highlights the benefits of a unified national ADMC program. According to Ben Mosier, executive director of HIWU, ongoing communication among laboratories facilitates the identification and resolution of discrepancies. Jeffrey A. Matty, Jr., executive director of the Pennsylvania Thoroughbred Horsemen’s Association, praised HIWU for its responsiveness and collaborative approach. Moving forward, continuous evaluation will ensure consistent and fair reporting of test results. The implementation of HISA Equine Analytical Laboratory accreditation further strengthens quality assurance within the industry.

The adoption of mass spectrometry as the standard method marks a significant step toward harmonization in total carbon dioxide content testing. It reflects a commitment to maintaining integrity and fairness in horseracing regulations. By addressing inconsistencies and fostering collaboration, HIWU and HISA are working together to benefit the entire industry, ensuring that all participants are treated equitably and that regulatory standards are consistently applied.